We are developing several experimental strategies and resources in different areas of life sciences. The resources are utilized to provide technical and research services.
1-Basic Techniques of Molecular Biology (practical and theoretical training)
- DNA, RNA, and protein extraction
- cDNA synthesis
- PCR
- Gel electrophoresis
- Gel elution
- Molecular cloning
- ELISA reader
2-Basic Tools of Bioinformatics (practical and theoretical training)
- Data basis to retrieve sequence of gene/mRNA and protein
- Primer designing (Manual, SnapGene)
- CRISPR design
- Primer testing (OligoAnalyzer)
- Phylogenetic tree (Mega 6)
3-Basic Techniques of Cell Biology (only demonstration and theoretical training)
- Cell culture
- Cell thawing
- Cell freezing
- MTT assay
- Colony formation assay
- Transfection and electroporation
4-Biochemical Assays, Invitro Assays (practical and theoretical training)
- Making plant extract
- Total phenolic contents
- Total flavonoid contents
- DPPH radical scavenging assay
- Antimicrobial assay
- Chromatography
5-Basic Techniques of Mouse Handling (practical and theoretical training)
- Mouse breeding
- IP dose
- IG dose
- Mouse necropsy
- Brief practice on mouse models of liver injury and diabetes
Hands-on Training Workshops
Designing and experimentation of CRISPR/Cas9 system 1000/day
Basic Tools of Bioinformatics in Life Sciences 1000/day
Laboratory Animal Handing and their Applications in Biomedical Research 1500/ 2 day
Hands-on Training Workshop on the Basic Tools of Molecular Biology 2000/ 2 day
Hands-on Training Workshop on the Basic Tools of Cell Biology 2500/ 2 day
Invitro antioxidant assays PKR 1000 / 2 days
Offered after approval from head office.
Scientific Material/ Services
- Albino mice Free (depends upon feasibility)
- Primer design and validation (Insilico) PKR 300/primer pair
- Reaction for PCR PKR 100/reaction
- Primer design for qPCR PKR 300/primer pair
- MTT assay PKR 500/sample
- Invivo trial for diabetes PKR 1,000/cage (5-6 mice)/24 days
- Invivo trial for liver injury PKR 1,000/cage (5-6 mice)/24 days
Comments are closed.