- Metabolism & Clinical Biochemistry 31 experiments
- Nutritional and Food Biochemistry 24 experiments
- Industrial Biochemistry 15 experiments
- Biochemical Techniques & Equipment 17 experiments
- Plant Biochemistry 20 experiments
- Scientific English Writing and Research Tools 14 exercises
Ordering Information
Price: 600/- PKR
Edition: 2023-2024 (1st edition)
ISBN: 978-627-7502-06-5 (Print)
This Series contains One hundred and twenty one (121) full length experiments
Published by Dr. Muhammad Ali
© Copyright 2022. All rights reserved by Dr. Muhammad Ali
Contact us for purchase and online ordering:
0320-7177763
[email protected]
Contents of EBB Series-III
Experiment 1.1- Estimation of serum glucose (Ortho-toluidine Method) 6
Experiment 1.2- Estimation of serum fatty acids 7
Experiment 1.3- Estimation of serum triglyceride 9
Experiment 1.4- Estimation of serum cholesterol (Sulphuric acid method) 10
Experiment 1.5- Estimation of serum/tissue cholesterol, HDL and LDL/VLDL 11
Experiment 1.6- Estimation of glycerol (Enzymatic method) 13
Experiment 1.7- Estimation of phospholipids by non-destructive method 15
Experiment 1.8- Blood group determination 17
Experiment 1.9- Estimation of blood hemoglobin 17
Experiment 1.10- Estimation of serum ammonia (Nesseler’s and Salicylate Methods) 19
Experiment 1.11- Estimation of urea in blood and serum samples 22
Experiment 1.12- Estimation of serum creatinine kinase (Jaffe’s reaction) 23
Experiment 1.13- Estimation of serum lactic acid 24
Experiment 1.14- Estimation of serum uric acid (Uricase method) 26
Experiment 1.15- Estimation of total serum proteins by Lowry assay 27
Experiment 1.16- Estimation of serum albumin 29
Experiment 1.17- Estimation of serum bilirubin (Malloy and Evelyn) 30
Experiment 1.18- Estimation of serum ALT (SGPT) 31
Experiment 1.19- Estimation of serum AST (SGOT) 33
Experiment 1.20- Estimation of serum ALP (Alkaline Phosphatase) 34
Experiment 1.21- Estimation of serum TAC (Total Antioxidant Capacity) 34
Experiment 1.22- Estimation of serum TOS (Total Oxidant Status) 36
Experiment 1.23- Estimation of serum GSH (mg of GSH/g of protein) 38
Experiment 1.24- Estimation of serum catalase 40
Experiment 1.25- Estimation of tissue MDA (Malondialdehyde) 41
Experiment 1.26- Estimation of cytotoxicity through hemolytic activity 43
Experiment 1.27- Estimation of Na, K, and Ca (in serum and urine) by flame photometer 44
Experiment 1.28- Estimation of inorganic phorphorous (P) in serum and urine 46
Experiment 1.29- Estimation of chloride in a urine sample by titration 48
Experiment 2.1- Determination of moisture contents of raisin 51
Experiment 2.2- Determination of ash content of cabbage 52
Experiment 2.3- Estimation of fat contents in almond 53
Experiment 2.4- Estimation of fat by chloroform–methanol procedure (Folch extraction) 54
Experiment 2.5- Protein quantification by Kjeldahl method 55
Experiment 2.6- Estimation of vitamin C in orange samples (mg /g of fruit) 56
Experiment 2.7- Preparation of different extracts from fennel seeds 57
Experiment 2.8- Determination of total phenolic contents (TPCs) 58
Experiment 2.9- Determination of total flavonoids contents (TFCs) 60
Experiment 2.10- Determination of reducing power 62
Experiment 2.11- DPPH radical scavenging activity 64
Experiment 2.12- Hydrogen peroxide scavenging capacity (phenol and 4-aminoantipyrine) 65
Experiment 2.13- Antioxidant activity in linoleic acid system (inhibition of lipid peroxidation) 68
Experiment 2.14- Estimation of ABTS cation radical scavenging activity 69
Experiment 2.15- Estimation of hydroxyl radical scavenging activity 71
Experiment 2.16- Estimation of ferric reducing antioxidant power (FRAP) 72
Experiment 2.17- Estimation of α-amylase inhibition assay 75
Experiment 2.18- Estimation of SOD (superoxide dismutase) activity 76
Experiment 2.19- Estimation of POD (peroxidase) activity 77
Experiment 2.20- Catalase activity (µM/mg of protein) 79
Experiment 2.21- Quantitation of citric acid 81
Experiment 2.22- Quantitation of succinic acid 82
Experiment 2.23- Antimicrobial activity of crude extract and standard drugs. 84
Experiment 2.24- Determination of minimum inhibitory concentration (MIC) 85
Experiment 3.1- Extraction of oil by Soxhlet method 88
Experiment 3.2- Determination of acid value 89
Experiment 3.3- Determination of iodine value (iodine monochloride) 89
Experiment 3.4- Determination of peroxide value 90
Experiment 3.5- Purification of phytochemicals by column chromatography 91
Experiment 3.6- Separation of phospholipids by TLC 91
Experiment 3.7- Determination of ethanol percentage in the fermentation broth 92
Experiment 3.9- Preservation of food by UV-radiation / chemical method 95
Experiment 3.11- Flow process diagram for sugar industry 95
Experiment 3.12- Flow process diagram for cooking oil industry 97
Experiment 3.13- Flow process diagram for fermentation and alcohol extraction industry 98
Experiment 3.13- Flow process diagram for waste-water treatment industry 98
Experiment 3.14- Flow process diagram for cloth industry 100
Experiment 3.15- Flow process diagram for soup industry 101
Experiment 4.1- Two-dimensional electrophoresis 103
Experiment 4.2- HPLC for the identification of specific phenolic compound 104
Experiment 4.3- LC-MS analysis of digested protein sample 105
Experiment 4.4- Flame photometry for the quantitation of alkali metals 106
Experiment 4.5- Atomic absorption spectroscopy for the quantitation of metal ions 107
Experiment 4.6- Inductively coupled plasma for simultaneous quantitation of multiple elements. 107
Experiment 4.7- Circular dichromic analysis for protein structure determination 108
Experiment 4.8- FTIR study 109
Experiment 4.9- General methods of fractionation of DNA and proteins 110
Experiment 4.10- Dialysis of blood 111
Experiment 4.11- Lypholysation 112
Experiment 4.13- Fluorescence spectroscopy 112
Experiment 4.14- X-Ray crystallography 112
Experiment 4.15- Scanning tunneling microscope (TEM) 113
Experiment 4.16- Scanning electron microscope (SEM) 114
Experiment 4.17- Native SDS-PAGE 115
Experiment 5.1- Sterilization and germination of tomato sees 117
Experiment 5.2- Embryo culture, anther culture 117
Experiment 5.3- Micro culture for double haploid 117
Experiment 5.4- Micropropagation by auxiliary buds 118
Experiment 5.5- Adventitious shoot proliferation 118
Experiment 5.6- Plant regeneration by organesis 119
Experiment 5.7- Somatic embryo genesis from culture cells 119
Experiment 5.8- Meristem culture for virus elimination, 120
Experiment 5.9- Invitro fertilization 120
Experiment 5.10- Protoplast isolation and culture 121
Experiment 5.11- Agrobacterium mediated transformation 121
Experiment 5.12- ELISA to certify pathogen free plant 122
Experiment 5.13- Extraction and qualitative analysis of chlorophyll 122
Experiment 5.14- Extraction and qualitative analysis of starch 122
Experiment 5.15- Extraction and qualitative analysis of lipids 123
Experiment 5.16- Extraction and qualitative analysis of auxins 123
Experiment 5.17- Extractions and estimation of alkaloids, phenolics and flavonoids 124
Experiment 5.18- Oxygen estimation by Wiklers method 124
Experiment 5.19- Estimation of amylases from germinating seeds 125
Experiment 5.20- Determination of water potential by Chardakov’s method. 125
Experiment 5.21- Plant expression vectors 126
Experiment 5.22- Plant transformation 126
Exercise 6.1- Websites for searching articles and books 128
Exercise 6.2- How to search a research or review article? 128
Exercise 6.3- What is general anatomy (parts) of a research article or dissertation? 132
Exercise 6.4- Guidelines for the reading of a research article? 133
Exercise 6.5- How to write a research article? 134
Exercise 6.6- General guidelines for the font and page set-up for thesis (dissertation) 135
Exercise 6.7- Parts of ppt for the defence of research article, synopsis, or thesis defence 136
Exercise 6.8- How to select a topic of research? 136
Exercise 6.9- A list of funding agencies 137
Exercise 6.10- How to write a good application for funding? 137
Exercise 6.11- How to use Endnote to insert citation? 141
Exercise 6.12- How to use GraphPad Prism for One-way ANOVA? 146
Exercise 6.13- How to use GraphPad Prism forTwo-way ANOVA? 150
Exercise 6.14- How to use GraphPad Prism to find correlation? 153
7.1- Reagents for Biochemistry, Molecular & Cell Biology lab 156
7.3- Composition of reagents for making gradient SDS page gel (6-17 %) 160
7.4- Buffers composition for plasmid extraction 160
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