Biochem-512 (List of Practical) 3(1-2)
- Preparation of different nature of solutions: (molar and normal, percentage). (S1: 2.1, 2.3, 2.7-2.9, 2.11, 2.12)
- Preparation of buffers of different molarity and pH. (S1: 2.22 to 2.24)
- Demonstration about diffusion, dialysis, Donnan’s equilibrium and osmosis. (S1: 2.25, 2.26)
- Chromatography: Paper chromatography. One dimensional two dimensional, (S1: 4.4, 6.9)
- circular and column chromatography. (S1: 4.8, 4.15)
- Carbohydrate analysis, Estimation of glucose by spectrophotometer (S1: 3.1 to 3.3, 3.6, 3.9, 3.12)
- True protein estimation by Biuret/Bradford and UV method. (S1: 4.10, 4.11, 4.14)
- Proximate analysis of foods and feed. (S1: 7.1 to 7.11)
- Estimation of vitamin-A and C. (S1: 7.1 to 7.11)
- Glycogen; Isolation and characterization. (S1: 3.16, 3.17)
Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1.
- Mid-term syllabus of Biochem-512: Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures, Laboratory glassware, (usually chapter 1 of experimental Biochemistry and biotechnology covers theory part of mid-exam) + molar and molal solutions…. (no calculations but general information about them)
…. PLUS…. First 150 MCQs from each of chapters 2, 3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.
Final exam Biochem-512: Standard procedures for preparation and storage of reagents. Buffers and solution chemistry. Theoretical background of chromatography. Importance of Biochemical analysis in plant and animal sciences. (usually chapter 1 of experimental Biochemistry and biotechnology plus principles of taught practical covers theory part of final exam)
…. PLUS…. First 150 MCQs from chapters 2,3,4 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.
Start preparing your presentations and assignments….. Assignment submission right after mid-exam. The presentations will also be started after mid-term exam.
__________________________________________________________________________________
Assignments and presentations of Biochem-512:
- The weightage of the assignment and presentation will be decided later.
- Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
- The presentations will also be started right after the mid-term exam.
- PPT file should not contain any designing/colours other than figures and diagrams.
- Slides should be in 16X9 ratio.
- Fonts of all header should be same and of all other text should be same.
- For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
- Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
- Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.
K. General Question to be asked during presentation
- 1) Pathway or importance of gene/technology/discovery
- 2) Rationale
- 3) Conclusion of the full article
- 4) Conclusion of every figure
- 5) New findings presented in the article
- 6) Applications of the findings
- 7) Future prospective
- 8) Applications of the work
- 9) Principle of every technique used in the research article
Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.
L. During Presentations, for EVERY FIGURE and graph, we may ask:
1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively
NO EXCUSE WILL BE ACCEPTED
_______________________________________________________________
Link to download the book ( MCQs in Biochemistry) is here.
Meeting ID: 316 824 288 013
Passcode: WNyjeA
Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.
2017-ag-9382 Haidar Iftikhar
2017-ag-10077 Ahmad Faraz
BIOCHEM-512 BIOCHEMICAL TECHNIQUES 3(1 -2)
Course Learning Outcomes
By the end of this course students will be able to;
1. Understand lab safety related knowledge and protocols, alongwith general lab tools and instruments
2. Related advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
3. Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
4. Analyse food and feed samples and learn electrophoresis
Theory
Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.
Practical
Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.
Suggested Readings
- Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
- Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
- Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
- Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
- Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
- Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
1-Biochem-512 M, Spring 25, Dr. M ALI (RIGHT AFTER MID-EXAM) | ||||
Sr. | Ag No. | Student’s Name | Group | Topic |
1 | 2022-ag-5240 | Tafseela Amiad | G1 | 1. Preparation of different nature of solutions: (molar and normal, percentage). (S1: 2.1, 2.3, 2.7-2.9, 2.11, 2.12) |
2 | 2022-ag-5241 | Alisha | ||
3 | 2022-ag-5242 | Ansa bibi | ||
4 | 2022-ag-5243 | Hadia Umar | ||
5 | 2022-ag-5245 | Ahmad Hassan | ||
6 | 2022-ag-5246 | Esha Arshad | ||
7 | 2022-ag-5247 | Asma Nasir | ||
8 | 2022-ag-5248 | Fariha Nawaz | ||
9 | 2022-ag-5249 | Esha Tahir | G2 | 2. Preparation of buffers of different molarity and pH. (S1: 2.22 to 2.24) |
10 | 2022-ag-5251 | Sahfa Sajjad | ||
11 | 2022-ag-5252 | Qirat Razzaq | ||
12 | 2022-ag-5253 | Kashaf Alam | ||
13 | 2022-ag-5254 | Aisha Ali | ||
14 | 2022-ag-5255 | Zainab | ||
15 | 2022-ag-5256 | Bushra Noor | ||
16 | 2022-ag-5257 | Mariyam | ||
17 | 2022-ag-5258 | Iqra batool | G3 | 3. Demonstration about diffusion, dialysis, Donnan’s equilibrium and osmosis. (S1: 2.25, 2.26) |
18 | 2022-ag-5259 | Manahil Younis | ||
19 | 2022-ag-5260 | Khuzaima Nadeem | ||
20 | 2022-ag-5261 | Ayesha Naveed | ||
21 | 2022-ag-5262 | Fiza Fatima | ||
22 | 2022-ag-5263 | Vaneeza Ahmad | ||
23 | 2022-ag-5264 | Hafsa Tahira | ||
24 | 2022-ag-5265 | M Muzazamal | ||
25 | 2022-ag-5266 | M Bilal | G4 | 4. Chromatography: Paper chromatography. One dimensional two dimensional, (S1: 4.4, 6.9) |
26 | 2022-ag-5267 | Abdul Hanan | ||
27 | 2022-ag-5268 | Faiza Laraib | ||
28 | 2022-ag-5269 | Saher Fraz | ||
29 | 2022-ag-5270 | M Usman | ||
30 | 2022-ag-5271 | Manahil Malik | ||
31 | 2022-ag-5272 | Maheen Asif | ||
32 | 2022-ag-5274 | Munim Ali | ||
33 | 2022-ag-5276 | Mubashar Ali | G5 | 5. circular and column chromatography. (S1: 4.8, 4.15) |
34 | 2022-ag-5277 | M Shehbaz Jameel | ||
35 | 2022-ag-5278 | Kisa Fatima | ||
36 | 2022-ag-5279 | M Mohsin Raza | ||
37 | 2022-ag-5280 | Kashaf Fatima | ||
38 | 2022-ag-5281 | Laina Azhar | ||
39 | 2022-ag-5282 | Aneela Shazain | ||
40 | 2022-ag-5283 | Rabia Ramazan | ||
41 | 2022-ag-5284 | Eman Fatima | G6 | 6. Carbohydrate analysis, Estimation of glucose by spectrophotometer (S1: 3.1 to 3.3, 3.6, 3.9, 3.12) |
42 | 2022-ag-5285 | Marium Saddiqua | ||
43 | 2022-ag-5286 | Ariba | ||
44 | 2022-ag-5287 | Marwa Nadeem | ||
45 | 2022-ag-5288 | Ghulam Hussain | ||
46 | 2022-ag-5289 | Samara Khalid | ||
47 | 2022-ag-5292 | Lubaba Saeed | ||
48 | 2022-ag-5293 | Nimra Eman | ||
49 | 2022-ag-5294 | Riza Sikandar | G7 | 7. True protein estimation by Biuret/Bradford and UV method. (S1: 4.10, 4.11, 4.14) |
50 | 2022-ag-5295 | Hamna Irshad | ||
51 | 2022-ag-5296 | Maryam Hamda | ||
52 | 2022-ag-5297 | Maryam Rahi | ||
53 | 2022-ag-5298 | Ali | ||
54 | 2022-ag-5299 | Haiqa Akhtar | ||
55 | 2022-ag-5300 | Saha Abbas | ||
56 | 2022-ag-5301 | Abdul Rehman | ||
57 | 2022-ag-5302 | Aiman Hamid | G8 | 8. Proximate analysis of foods and feed. (S1: 7.1 to 7.11) |
58 | 2022-ag-5303 | Marium Fatima | ||
59 | 2022-ag-5304 | Malaika Nadeem | ||
60 | 2022-ag-5306 | Saba Javed | ||
61 | 2022-ag-5307 | Marriam Saleem | ||
62 | 2022-ag-5308 | Afsheen Qadri | ||
63 | 2022-ag-5309 | Itrat Fatima | ||
64 | 2022-ag-5311 | Maha | ||
65 | 2022-ag-5312 | Zeeshan | G9 | 9. Estimation of vitamin-A and C. (S1: 7.1 to 7.11) |
66 | 2022-ag-5313 | Farwa Asghar | ||
67 | 2022-ag-5314 | Umer Akmal | ||
68 | 2022-ag-5315 | Tayyaba Eman | ||
69 | 2022-ag-5316 | Daniya Pakeeza | ||
70 | 2022-ag-5317 | Tayyaba Ali | ||
71 | 2022-ag-5318 | Aiman Shabir | ||
72 | 2022-ag-5319 | Tayyaba Naveed | ||
73 | 2022-ag-7031 | Ayesha Aslam | G10 | 10. Glycogen; Isolation and characterization. (S1: 3.16, 3.17) |
74 | 2022-ag-5365 | Sabhat | ||
75 | 2022-ag-5355 | Ifra | ||
76 | 2022-ag-5320 | Fatima Idrees | ||
77 | 2022-ag-5338 | Aleeza | ||
78 | 2022-ag-5364 | Fatima Meraj | ||
2-Biochem-512 E, Spring 25, Dr. M ALI (RIGHT AFTER MID-EXAM) | ||||
Sr. | Ag No. | Student’s Name | Group | Topic |
1 | 2022-ag-5321 | Kashmala javed | G1 | 1. Preparation of different nature of solutions: (molar and normal, percentage). (S1: 2.1, 2.3, 2.7-2.9, 2.11, 2.12) |
2 | 2022-ag-5322 | M.Hashim | ||
3 | 2022-ag-5323 | Sumaiya Mazhar | ||
4 | 2022-ag-5324 | Amna Naz | ||
5 | 2022-ag-5325 | Ayesha Saeed | G2 | 2. Preparation of buffers of different molarity and pH. (S1: 2.22 to 2.24) |
6 | 2022-ag-5326 | M.Moiz | ||
7 | 2022-ag-5328 | Amna Bibi | ||
8 | 2022-ag-5329 | Tehzeeb Raza | ||
9 | 2022-ag-5330 | Mehak Khalid | G3 | 3. Demonstration about diffusion, dialysis, Donnan’s equilibrium and osmosis. (S1: 2.25, 2.26) |
10 | 2022-ag-5331 | Maria Sadiq | ||
11 | 2022-ag-5332 | Khizra Azhar | ||
12 | 2022-ag-5333 | Sehrish Fatima | ||
13 | 2022-ag-5334 | Sehar Saif | G4 | 4. Chromatography: Paper chromatography. One dimensional two dimensional, (S1: 4.4, 6.9) |
14 | 2022-ag-5335 | Aqsa | ||
15 | 2022-ag-5336 | Shamkha Asif | ||
16 | 2022-ag-5339 | Farah Yaseen | ||
17 | 2022-ag-5341 | Nimra Rauf | G5 | 5. circular and column chromatography. (S1: 4.8, 4.15) |
18 | 2022-ag-5342 | Awais Pitras | ||
19 | 2022-ag-5343 | Laiba Shafique | ||
20 | 2022-ag-5344 | Khadija Aslam | ||
21 | 2022-ag-5345 | Eman Aftab | G6 | 6. Carbohydrate analysis, Estimation of glucose by spectrophotometer (S1: 3.1 to 3.3, 3.6, 3.9, 3.12) |
22 | 2022-ag-5346 | Minahil Zafar | ||
23 | 2022-ag-5347 | M.Bilal | ||
24 | 2022-ag-5348 | Zimal Imran | ||
25 | 2022-ag-5349 | Abdul Haseeb | G7 | 7. True protein estimation by Biuret/Bradford and UV method. (S1: 4.10, 4.11, 4.14) |
26 | 2022-ag-5350 | Hadia Talib | ||
27 | 2022-ag-5351 | Tawasaal Zahra | ||
28 | 2022-ag-5353 | Ayesha Sattar | ||
29 | 2022-ag-5356 | Uswa Mustafa | G8 | 8. Proximate analysis of foods and feed. (S1: 7.1 to 7.11) |
30 | 2022-ag-5357 | Hooria Naeem | ||
31 | 2022-ag-5359 | Nighat Yousaf | ||
32 | 2022-ag-5360 | Aniqa ilyas | ||
33 | 2022-ag-5362 | Maham Baig | G9 | 9. Estimation of vitamin-A and C. (S1: 7.1 to 7.11) |
34 | 2022-ag-5363 | Tayyeba Sarwar | ||
35 | 2022-ag-5366 | Maryam Umer | ||
36 | 2022-ag-5367 | Mubara Iqbal | ||
37 | 2022-ag-5368 | Ayat Salam | G10 | 10. Glycogen; Isolation and characterization. (S1: 3.16, 3.17) |
38 | 2022-ag-5369 | Tayyeba Sehar | ||
39 | 2022-ag-5370 | M.Nouman | ||
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