Biochem-512 (List of Practical) 3(1-2)

  1. Preparation of different nature of solutions:  (molar and normal, percentage). (S1: 2.1, 2.3, 2.7-2.9, 2.11, 2.12)
  2. Preparation of buffers of different molarity and pH. (S1: 2.22 to 2.24)
  3. Demonstration about diffusion, dialysis, Donnan’s equilibrium and osmosis. (S1: 2.25, 2.26)
  4. Chromatography: Paper chromatography. One dimensional two dimensional, (S1: 4.4, 6.9)
  5. circular and column chromatography.  (S1: 4.8, 4.15)
  6. Carbohydrate analysis, Estimation of glucose  by spectrophotometer (S1: 3.1 to 3.3, 3.6, 3.9, 3.12)
  7. True protein estimation by Biuret/Bradford and UV method.  (S1: 4.10, 4.11, 4.14)
  8. Proximate analysis of foods and feed. (S1: 7.1 to 7.11)
  9. Estimation of vitamin-A and C. (S1: 7.1 to 7.11)
  10. Glycogen; Isolation and characterization. (S1: 3.16, 3.17)

Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.

  1. Mid-term syllabus of Biochem-512: Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures, Laboratory glassware,  (usually chapter 1 of experimental Biochemistry and biotechnology covers theory part of mid-exam) + molar and molal solutions…. (no calculations but general information about them)

…. PLUS…. First 150 MCQs from each of chapters 2, 3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

Final exam Biochem-512: Standard procedures for preparation and storage of reagents. Buffers and solution chemistry. Theoretical background of chromatography. Importance of Biochemical analysis in plant and animal sciences.  (usually chapter 1 of experimental Biochemistry and biotechnology plus principles of taught practical covers theory part of final exam)

 …. PLUS…. First 150 MCQs from chapters 2,3,4 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

Start preparing your presentations and assignments….. Assignment submission right after mid-exam. The presentations will also be started after mid-term exam.

                            __________________________________________________________________________________

Assignments and presentations of Biochem-512: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

K. General Question to be asked during presentation

  1. 1) Pathway or importance of gene/technology/discovery
  2. 2) Rationale
  3. 3) Conclusion of the full article
  4. 4) Conclusion of every figure
  5. 5) New findings presented in the article
  6. 6) Applications of the findings
  7. 7) Future prospective
  8. 8) Applications of the work
  9. 9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

NO EXCUSE WILL BE ACCEPTED

 _______________________________________________________________

Link to download the book ( MCQs in Biochemistry) is here.


_______________________________________________________________
 
Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 316 824 288 013
Passcode: WNyjeA

 __________________________________________________________________________________
 

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

BIOCHEM-512                  BIOCHEMICAL TECHNIQUES                  3(1 -2)

 Course Learning Outcomes

By the end of this course students will be able to;

1.    Understand lab safety related knowledge and protocols, alongwith general lab tools and instruments

2.    Related advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules

3.    Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.

4.    Analyse food and feed samples and learn electrophoresis

 

Theory

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

 

Practical

Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.

 

Suggested Readings

  1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
  2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
  3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
  4. Shawn, O.F.  and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
  5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
  6. Wilson, K. and J. Walker. 2018.  Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.
  8. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
  9. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
MMGL
This is template... Try to write, whatever headings are possible for a practical.
Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.
1-Biochem-512 M, Spring 25, Dr. M ALI  (RIGHT AFTER MID-EXAM)
Sr. Ag No. Student’s Name Group Topic
1 2022-ag-5240 Tafseela Amiad G1 1. Preparation of different nature of
solutions:  (molar and normal,
percentage). (S1: 2.1, 2.3, 2.7-2.9, 2.11, 2.12)
2 2022-ag-5241 Alisha 
3 2022-ag-5242 Ansa bibi 
4 2022-ag-5243 Hadia Umar
5 2022-ag-5245 Ahmad Hassan 
6 2022-ag-5246 Esha Arshad
7 2022-ag-5247 Asma Nasir
8 2022-ag-5248 Fariha Nawaz
9 2022-ag-5249 Esha Tahir G2 2. Preparation of buffers of different molarity
and pH. (S1: 2.22 to 2.24)
10 2022-ag-5251 Sahfa Sajjad
11 2022-ag-5252 Qirat Razzaq
12 2022-ag-5253 Kashaf Alam 
13 2022-ag-5254 Aisha Ali
14 2022-ag-5255 Zainab
15 2022-ag-5256 Bushra Noor
16 2022-ag-5257 Mariyam
17 2022-ag-5258 Iqra batool G3 3. Demonstration about diffusion, dialysis,
Donnan’s equilibrium and osmosis. (S1: 2.25, 2.26)
18 2022-ag-5259 Manahil Younis 
19 2022-ag-5260 Khuzaima Nadeem
20 2022-ag-5261 Ayesha Naveed
21 2022-ag-5262 Fiza Fatima
22 2022-ag-5263 Vaneeza Ahmad
23 2022-ag-5264 Hafsa Tahira 
24 2022-ag-5265 M Muzazamal
25 2022-ag-5266 M Bilal G4 4. Chromatography: Paper chromatography. One
dimensional two dimensional, (S1: 4.4, 6.9)
26 2022-ag-5267 Abdul Hanan
27 2022-ag-5268 Faiza Laraib
28 2022-ag-5269 Saher Fraz
29 2022-ag-5270 M Usman
30 2022-ag-5271 Manahil Malik
31 2022-ag-5272 Maheen Asif
32 2022-ag-5274 Munim Ali
33 2022-ag-5276 Mubashar Ali  G5 5. circular and column chromatography.  (S1: 4.8, 4.15)
34 2022-ag-5277 M Shehbaz Jameel
35 2022-ag-5278 Kisa Fatima
36 2022-ag-5279 M Mohsin Raza
37 2022-ag-5280 Kashaf Fatima
38 2022-ag-5281 Laina Azhar
39 2022-ag-5282 Aneela Shazain
40 2022-ag-5283 Rabia Ramazan
41 2022-ag-5284 Eman Fatima G6 6. Carbohydrate analysis, Estimation of
glucose  by spectrophotometer (S1: 3.1
to 3.3, 3.6, 3.9, 3.12)
42 2022-ag-5285 Marium Saddiqua 
43 2022-ag-5286 Ariba
44 2022-ag-5287 Marwa Nadeem 
45 2022-ag-5288 Ghulam Hussain
46 2022-ag-5289 Samara Khalid
47 2022-ag-5292 Lubaba Saeed
48 2022-ag-5293 Nimra Eman
49 2022-ag-5294 Riza Sikandar G7 7. True protein estimation by Biuret/Bradford
and UV method.  (S1: 4.10, 4.11, 4.14)
50 2022-ag-5295 Hamna Irshad
51 2022-ag-5296 Maryam Hamda 
52 2022-ag-5297 Maryam Rahi
53 2022-ag-5298 Ali
54 2022-ag-5299 Haiqa Akhtar
55 2022-ag-5300 Saha Abbas
56 2022-ag-5301 Abdul Rehman
57 2022-ag-5302 Aiman Hamid  G8 8. Proximate analysis of foods and feed. (S1:
7.1 to 7.11)
58 2022-ag-5303 Marium Fatima
59 2022-ag-5304 Malaika Nadeem
60 2022-ag-5306 Saba Javed
61 2022-ag-5307 Marriam Saleem
62 2022-ag-5308 Afsheen Qadri 
63 2022-ag-5309 Itrat Fatima
64 2022-ag-5311 Maha
65 2022-ag-5312 Zeeshan G9 9. Estimation of vitamin-A and C. (S1: 7.1 to
7.11)
66 2022-ag-5313 Farwa Asghar
67 2022-ag-5314 Umer Akmal
68 2022-ag-5315 Tayyaba Eman
69 2022-ag-5316 Daniya Pakeeza 
70 2022-ag-5317 Tayyaba Ali
71 2022-ag-5318 Aiman Shabir
72 2022-ag-5319 Tayyaba Naveed
73 2022-ag-7031 Ayesha Aslam G10 10. Glycogen; Isolation and characterization.
(S1: 3.16, 3.17)
74 2022-ag-5365 Sabhat 
75 2022-ag-5355 Ifra
76 2022-ag-5320 Fatima Idrees
77 2022-ag-5338 Aleeza
78 2022-ag-5364 Fatima Meraj
     
     
2-Biochem-512 E, Spring 25, Dr. M ALI (RIGHT AFTER MID-EXAM)
Sr. Ag No. Student’s Name Group Topic
1 2022-ag-5321 Kashmala javed G1 1.
Preparation of different nature of solutions: 
(molar and normal, percentage). (S1: 2.1, 2.3, 2.7-2.9, 2.11, 2.12)
2 2022-ag-5322 M.Hashim 
3 2022-ag-5323 Sumaiya Mazhar 
4 2022-ag-5324 Amna Naz
5 2022-ag-5325 Ayesha Saeed  G2 2. Preparation of
buffers of different molarity and pH. (S1: 2.22 to 2.24)
6 2022-ag-5326 M.Moiz
7 2022-ag-5328 Amna Bibi
8 2022-ag-5329 Tehzeeb Raza
9 2022-ag-5330 Mehak Khalid G3 3. Demonstration about
diffusion, dialysis, Donnan’s equilibrium and osmosis. (S1: 2.25, 2.26)
10 2022-ag-5331 Maria Sadiq
11 2022-ag-5332 Khizra Azhar
12 2022-ag-5333 Sehrish Fatima 
13 2022-ag-5334 Sehar Saif  G4 4. Chromatography:
Paper chromatography. One dimensional two dimensional, (S1: 4.4, 6.9)
14 2022-ag-5335 Aqsa
15 2022-ag-5336 Shamkha Asif
16 2022-ag-5339 Farah Yaseen
17 2022-ag-5341 Nimra Rauf G5 5. circular and column
chromatography.  (S1: 4.8, 4.15)
18 2022-ag-5342 Awais Pitras 
19 2022-ag-5343 Laiba Shafique
20 2022-ag-5344 Khadija Aslam
21 2022-ag-5345 Eman Aftab G6 6. Carbohydrate
analysis, Estimation of glucose  by
spectrophotometer (S1: 3.1 to 3.3, 3.6, 3.9, 3.12)
22 2022-ag-5346 Minahil Zafar
23 2022-ag-5347 M.Bilal 
24 2022-ag-5348 Zimal Imran
25 2022-ag-5349 Abdul Haseeb G7 7. True protein
estimation by Biuret/Bradford and UV method. 
(S1: 4.10, 4.11, 4.14)
26 2022-ag-5350 Hadia Talib
27 2022-ag-5351 Tawasaal Zahra
28 2022-ag-5353 Ayesha Sattar
29 2022-ag-5356 Uswa Mustafa G8 8. Proximate analysis
of foods and feed. (S1: 7.1 to 7.11)
30 2022-ag-5357 Hooria Naeem
31 2022-ag-5359 Nighat Yousaf
32 2022-ag-5360 Aniqa ilyas
33 2022-ag-5362 Maham Baig  G9 9. Estimation of
vitamin-A and C. (S1: 7.1 to 7.11)
34 2022-ag-5363 Tayyeba Sarwar
35 2022-ag-5366 Maryam Umer
36 2022-ag-5367 Mubara Iqbal
37 2022-ag-5368 Ayat Salam G10 10. Glycogen;
Isolation and characterization. (S1: 3.16, 3.17)
38 2022-ag-5369 Tayyeba Sehar
39 2022-ag-5370 M.Nouman
     

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