Biochem-605, Molecular Biotechnology
(list of practical) 3(2-1)

  1. Isolation of genomic DNA from any biological sample; (1.4)
  2. Detection/confirmation of DNA via agarose gel electrophoresis;(1.11)
  3. Quantification of DNA/RNA via spectrophotometer;(1.7)
  4. Designing of Primers for Polymerase chain reaction;(1.8, 1.9)
  5. Polymerase chain reaction/amplification of a specific gene via thermocycler;(1.10)
  6. Digestion of DNA with restriction enzymes or PCR-RFLP. (4.5)

 Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023, 1st edition, ISBN: 978-969-23317-2-2.

Biochem-605 (Mid-term syllabus) 3(3-0): 

Biotechnology and Molecular Biotechnology; Recombinant Protein Production; Gene isolation with PCR and RT-PCR; Agarose gel electrophoresis; Cloning in Plasmid: Plasmid preparation, restriction digestion; Screening of recombinant clones. DNA sequencing

PLUS………. First 150 MCQs from chapter 9 of the book ” MCQs in Biochemistry”.

Biochem-605 (After mid-term exam):

 Heterologous expression of genes in bacteria and yeast; Recombinant protein isolation and purification; Antibodies and proteins for the study of protein expression; Transgenic animals and plants (GMOs); Gene therapy (CRISPER-CAS); Vaccine production using biotechnology. 

……PLUS………. First 150 MCQs from chapter 9 of the book ” MCQs in Biochemistry”.

……PLUS……… Final exam will contain 30% from mid syllabus.

Biochem-605: Start preparing your presentations and assignments….. Presentations and Assignment will be taken in first class right after mid-term exam. The presentations will also be started right after mid-term exam.

                                       __________________________________________________________________________________

Assignments and presentations: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

K. General Question to be asked during presentation

  1. 1) Pathway or importance of gene/technology/discovery
  2. 2) Rationale
  3. 3) Conclusion of the full article
  4. 4) Conclusion of every figure
  5. 5) New findings presented in the article
  6. 6) Applications of the findings
  7. 7) Future prospective
  8. 8) Applications of the work
  9. 9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

NO EXCUSE WILL BE ACCEPTED

______________________________________________________________________________

MCQs will appear from text-book and from chapter 2 of the book ” MCQs in Biochemistry”.

Link to download this book is given below:

https://b-ok.cc/dl/1055171/2292c5

______________________________________________________________________________

Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 316 824 288 013
Passcode: WNyjeA

______________________________________________________________________________

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number. 

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Farazesentaino

 

Link to download the book ( MCQs in Biochemistry) is here.

 

BIOCHEM-605       Molecular Biotechnology       3(2-1)

Course Learning Outcomes

By the end of this course students will be able to;

 

  1. Demonstrate molecular biotechnology.
  2. Explain basic concepts in practical aspects of recombinant DNA technology.
  3. Demonstrate with advancements in applied biotechnology.

Theory

Biotechnology and Molecular Biotechnology; Recombinant Protein Production; Gene isolation with PCR and RT-PCR; Agarose gel electrophoresis; Cloning in Plasmid: Plasmid preparation, restriction digestion; Screening of recombinant clones. DNA sequencing; Heterologous expression of genes in bacteria and yeast; Recombinant protein isolation and purification; Antibodies and proteins for the study of protein expression; Transgenic animals and plants (GMOs); Gene therapy (CRISPER-CAS); Vaccine production using biotechnology.

Practical

Isolation of genomic DNA from any biological sample; Detection/confirmation of DNA via agarose gel electrophoresis; Quantification of DNA/RNA via spectrophotometer; Designing of Primers for Polymerase chain reaction; Polymerase chain reaction/amplification of a specific gene via thermocycler; Digestion of DNA with restriction enzymes or PCR-RFLP.

Suggested Readings

  1. Brown, T. 2016. Gene Cloning and DNA Analysis. 7th Ed. Blackwell Scientific Publications, Australia.
  2. Glick, B.R. and J.J. Pasternack. 2003. Molecular Biotechnology. 3rd Ed. American Society of Microbiology Press, USA.
  3. Nelson, D.L. and M.M. Cox. 2021. Lehninger’s Principles of Biochemistry. 8th Ed. WH Freeman & Company, USA.
  4. Primrose, S.B. and R.M. Twyman. 2016. Principles of Gene Manipulation and Genomics. 8th Ed. Blackwell Scientific Publications, Australia.
  5. Recent research papers on relevant contents.
  6. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2019. 1st edition, ISBN: 978-969-23317-2-2.
  8. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2023, 1st edition, ISBN: 978-969-23317-6-0 
MMGL- Life sciences
Life Sciences by Dr. Muhammad Ali
Biochem-605, BS Biochem, 5TH, M-Winter 2023, Dr. Muhammad Ali; 28-08-2023
Sr.Ag No.Student’s NameGroupResearch article for presentation and 1 page summary
12021-ag-8431Eisha Zahid G1Emodin attenuates high lipid-induced liver metastasis through the AKT and ERK pathways in vitro in breast cancer cells and in a mouse xenograft model
22021-ag-8441Linta Shafique
32021-ag-8452Muhammad Haris
42021-ag-8458Muhammad Ramzan
52021-ag-8463Nimra Shoukat
62021-ag-8466Noor-Ul-AinG2PRMT6 Regulates RAS/RAF Binding and MEK/ERKMediated Cancer Stemness Activities in Hepatocellular Carcinoma through CRAF Methylation
72021-ag-8472Saliha Nawaz
82021-ag-8484Abdul Qadeer
92021-ag-8485Aleena Khalid
102021-ag-8486Aleeza Tahir
112021-ag-8487Ali RazaG3Impaired LXRa Phosphorylation Attenuates Progression of Fatty Liver Disease
122021-ag-8488Amna Hamid
132021-ag-8490Anooma
142021-ag-8491Ansa Sehar
152021-ag-8492Areej Fatima Ikram
162021-ag-8493Asnan SaleemG4Aldolase B-Mediated Fructose Metabolism Drives Metabolic Reprogramming of Colon Cancer Liver Metastasis
172021-ag-8494Ayesha Hassan
182021-ag-8495Ayesha Tahir
192021-ag-8496Esha Batool
202021-ag-8497Esha Mujahid
212021-ag-8498Fatima HaiderG5Treatment of acromegaly by rosiglitazone via upregulating 15-PGDH in both pituitary adenoma and liver
222021-ag-8499Fatima Tahir
232021-ag-8500Ghazia Ashraf
242021-ag-8501Hadia Akram
252021-ag-8502Hafsa Areej
262021-ag-8503Iman FatimaG6In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target
272021-ag-8504Iman Fatima
282021-ag-8505Iqra Javed
292021-ag-8507Kashaf E Noor
302021-ag-8509Khadija Fatima
312021-ag-8510Kiran ArshadG7Selective Surface PEGylation of UiO-66 Nanoparticles for Enhanced Stability, Cell Uptake, and pH-Responsive Drug Delivery
322021-ag-8511Mahnoor
332021-ag-8512Mahnoor Fatima
342021-ag-8513Manahil Kashif
352021-ag-8514Maryam Naseer
362021-ag-8515MinahilG8Preventing Allograft Rejection by Targeting Immune Metabolism
372021-ag-8517M.Ahtesham Muhedeen
382021-ag-8518M. Ehtisham Basel
392021-ag-8521Muhammad Shahzad
402021-ag-8525Muqaddas Raza
412021-ag-8526Naila NawazG9Alteration of Tumor Metabolism by CD4+ T Cells Leads to TNF-a-Dependent Intensification of Oxidative Stress and Tumor Cell Death
422021-ag-8527Namal Rauf
432021-ag-8528Nimra Akram
442021-ag-8529Noor Fatima
452021-ag-8530Noor Fatima
462021-ag-8531Noreen AyeshaG10Controlling cancer-induced inflammation with a nucleic acid scavenger prevents lung metastasis in murine models of breast cancer
472021-ag-8532Rafia Zulfiqar
482021-ag-8533Sadaqat Ali
492021-ag-8535Toima Riaz
50  

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