Biochem-509

BIOCHEM-509      Enzyme Biotechnology      4(0-4)

 Course learning outcomes

By the end of this course students will be able to;

1. 1. Educate students about recent advancements in enzymology.
2. 2. Explain the skills of development of enzyme based products.
3. 3. Describe the applications of enzymes in various fields of life.

 Practical

Isolation of enzymes from plant/animal sources; Microbial technology of enzymes: Production of enzyme(s) and enzyme assay; Hyperproduction of enzymes through physical/chemical mutagenesis and chemical modifications; Isolation of enzymes by ammonium sulfate precipitation; Purification of enzymes by ion exchange and gel filtration chromatography; Effect of pH on enzyme activity; Effect of temperature on enzyme activity; Effect of substrate concentration and determination of Km and Vmax; Effect of cofactors and metal ions on enzyme activity; Effect of inhibitors on enzyme activity; Immobilization of enzyme(s) using different supports and techniques; SDS-PAGE; Industrial Visits.

Suggested Readings

1. 1. Buchholz, K., V. Kasche, U.T. Bornscheuer. 2012. Biocatalysts and Enzyme Technology. John Wiley & Sons, USA.
2. 2. Chatterjea, M.N. and R. Shinde. 2012. Textbook of Medical Biochemistry. 8th Ed. Jaypee Brothers, Medical Publishers (P) Ltd, New Delhi, India.
3. 3. Gray, N., M. Calvin and S.C. Bhatia. 2012. Enzymes Biotechnology. CBS Publishers, India.
4. 4. Kermasha,S. and M.N.A. Eskin. 2020. Enzymes: Novel Biotechnological Approaches for the Food Industry. 1st Ed. Academic Press, USA.
5. 5. Kuddus, M. and C. Aguilar. 2021. Value-Addition in Food Products and Processing Through Enzyme Technology. Academic Press, USA.
6. 6. Shukla, P. and B.I. Pletschke. (Eds.). 2013. Advances in Enzyme Biotechnology. Springer, Germany.
7.  7. Zia, M.A. 2022. Manual of Enzyme Biotechnology. MAS Publishers, Faisalabad, Pakistan.