Biochem-516

Biochem-516 Enzyme Kinetics 3(3-0)

Mid-term syllabus of Biochem-516, 3(3-0):

Chemical kinetics and rate equations, order of reaction, reaction rates, rate constants and steady states. Enzyme kinetics and its importance in the study of enzymes and mechanisms of enzyme catalyzed reactions, types of enzyme catalyzed reactions; Free energy of activation and effect of enzymes; Catalytic mechanisms; types of catalysis contributing to enzyme efficiency, catalytic groups involved in catalysis; Michaelis-Menten equation and its application to one and two substrate enzyme catalyzed reactions; 

…. PLUS…. Fist 150 MCQs from chapters 6 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

 Final syllabus of Biochem-516:

The mid-syllabus will be 25%

…. PLUS…. Transformations of Michaelis-Menton equation; Uses of Lineweaver-Burk plot and Eddie Hofstee plots for the determination of Vmax and KM; Effect of pH, temperature and inhibitors; Thermodynamic studies and kinetic models of enzymes Study of thermo-stability and half-life of enzymes; Kinetics of competitive, non-competitive, uncompetitive and mixed inhibition; Non-Michaelis-Menten Kinetics; Types of enzyme activity assays; Types of enzyme activity units and their relationships; Significance of enzyme kinetics in clinical and industrial applications of enzymes.

…. PLUS…. All MCQs from chapter 6 (Enzymes) of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.

 Assignemnts and presentations of Biochem-516: 

1. The weightage of the assignment and presentation will be decided later. 
2. Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
3. The presentations will also be started right after the mid-term exam.
4. PPT file should not contain any designing/colours other than figures and diagrams.
5. Slides should be in 16X9 ratio.
6. Fonts of all header should be same and of all other text should be same. 
7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

NO EXCUSE WILL BE ACCEPTED

K. General Question to be asked during presentation

1) Pathway or importance of gene/technology/discovery

2) Rationale

3) Conclusion of the full article

4) Conclusion of every figure

5) New findings presented in the article

6) Applications of the findings

7) Future prospective

8) Applications of the work

9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

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Link to download the book ( MCQs in Biochemistry) is given below:

https://b-ok.cc/dl/1055171/2292c5

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